Attune Cytometer
Attune Cytometer - Related Videos
11:00
Live Cell Cycle Analysis of Drosophila Tissues using the Attune Acoustic Focusing Cytometer and Vybrant DyeCycle Violet DNA Stain
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Cited by 13 •
2013
A protocol for cell cycle analysis of live Drosophila tissues using the Attune Acoustic Focusing Cytometer is described. This protocol simultaneously provides information about relative cell size, cell number, DNA content and cell type via lineage tracing or tissue specific expression of fluorescent proteins in vivo.
10:58
Assessment of GFP Expression and Viability Using the Tali Image-Based Cytometer
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Cited by 14 •
2011
This protocol describes how to perform cell viability and fluorescence expression assays using the Tali Image-Based Cytometer.
10:59
Mass Cytometry: Protocol for Daily Tuning and Running Cell Samples on a CyTOF Mass Cytometer
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Cited by 48 •
2012
The steps necessary for daily tuning and optimization of the performance of a CyTOF mass cytometer are described. Comments on optimal sample preparation and flow rate are...
05:43
Determination of Continuity Index Values in Atrial Fibrillation Ablation with Proactive Esophageal Cooling
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Cited by 6 •
2024
This protocol describes the methods used to determine the continuity index in patients undergoing pulmonary vein isolation procedures using radiofrequency ablation and demonstrates the differences in continuity index between ablation procedures using proactive esophageal cooling as compared to procedures using traditional luminal esophageal temperature monitoring.
13:59
Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology
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Cited by 9 •
2014
Spaceflight blood diagnostics need innovation. Few demonstrations have been published illustrating in-flight, reduced-gravity health diagnostic technology. Here we present a method for construction and operation of a parabolic flight test rig for a prototype point-of-care flow-cytometry design, with components and preparation strategies adaptable to other setups.
FAQs
Acoustic focusing aligns cells within a fluid stream before they pass through the laser beams. This controlled alignment helps the instrument examine cells consistently and associate their light signals with individual cellular events. The resulting measurements support rapid analysis of cell populations while reducing variability caused by cells passing through the detection region in less predictable positions.
Light scatter provides information related to cellular features, whereas fluorescence reports signals from labeled molecules or other fluorescent components. Examining these signal types together lets researchers distinguish cell populations and assess biological characteristics through more than one measurement. This combination is especially useful when identifying cellular differences or tracking specific protein-expression patterns.
Multiparameter detection allows several cellular characteristics to be measured from the same analytical process rather than relying on one signal alone. Researchers can therefore compare population features with fluorescence-based measurements and obtain a more detailed profile of biological samples. This broader information supports studies of cell identity, viability, apoptosis, cell cycle, and protein expression.
Different experimental responses can produce different patterns in scatter or fluorescence measurements. By examining these signals across individual cells, researchers can evaluate changes in viability, apoptosis, cell-cycle state, or protein expression within a population. This approach helps reveal whether an experimental condition affects most cells similarly or produces distinct subpopulations with different biological characteristics.
A sample is processed as cells move through a fluid stream, become aligned by acoustic focusing, and pass through laser beams. Detectors then record light scatter and fluorescence, and the resulting signals are used to examine cellular characteristics and populations. This workflow combines efficient sample processing with multiparameter measurement for quantitative biological analysis.
Researchers may select the instrument when they need rapid, quantitative information about cell populations or cellular responses. Supported applications include immunophenotyping, viability and apoptosis analysis, cell-cycle studies, and protein-expression measurements. These uses make it relevant to experiments comparing biological samples or evaluating how cells respond to defined experimental conditions.
The analysis can provide quantitative measurements of cell populations and biological characteristics at the individual-cell level. Depending on the study, results may describe population differences, viability or apoptosis patterns, cell-cycle distributions, or protein-expression changes. Such outcomes help researchers evaluate cellular states and compare responses across samples or experimental conditions.
In basic biology, the system supports detailed investigation of cell states and population behavior. In disease studies, the same measurements can help characterize altered cellular features or responses. Its ability to combine rapid processing with multiparameter detection also supports evaluation of experimental treatments or conditions, linking measured cellular changes to broader biological questions.