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JoVE Journal Neuroscience
Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures
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Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures

Article DOI: 10.3791/50253-v • 08:13 min • March 11th, 2013
March 11th, 2013 •
1, 1, 2, 1
1Department of Physiology, David Geffen School of Medicine at UCLA, 2Natural Science Division, Pepperdine University

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Summary
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This video demonstrates the procedures used to grow primary cultures of embryonic Xenopus nerve and muscle cells and the usefulness of this preparation for making simultaneous pre- and post-synaptic patch clamp recordings.

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Simultaneous Recording Pre-synaptic Post-synaptic Electrophysiological Xenopus Nerve-muscle Co-cultures Coupling Of Ionic Currents Neurotransmitter Release Invertebrate Preparations Squid Giant Synapse Vertebrate Preparations Embryonic Xenopus Motoneurons Muscle Cells Functional Synapses Nerve And Muscle Cell Development Synaptic Interactions Co-cultures Advantages Biophysical Properties Of Ion Channels Regulation Of Transmitter Release Neurite Outgrowth Synaptogenesis Molecular Mechanisms Of Neurotransmitter Release Diffusible Messengers In Neuromodulation In Vitro Synaptic Plasticity
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