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An Injury Paradigm to Investigate Central Nervous System Repair in Drosophila

Article DOI: 10.3791/50306-v • 10:49 min • March 28th, 2013
March 28th, 2013 •
1, 1
1Neurodevelopment Group, School of Biosciences, University of Birmingham

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An injury paradigm using the Drosophila larval ventral nerve cord to investigate central nervous system regeneration and repair is described. Stabbing followed by laser scanning confocal microscopy in time-lapse and fixed specimens, combined with quantitative analysis with purposefully developed software and genetics, are used to investigate the molecular mechanisms of CNS regeneration and repair.

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Injury Paradigm Central Nervous System Repair Drosophila Cellular Responses CNS Injury Regeneration Medical Neuroscience Genetic Toolkit Lesion Ventral Nerve Cord (VNC) Tungsten Needle Time-lapse Filming Laser Scanning Confocal Microscopy Neuron Glial Cells Immunofluorescence Staining Cell Morphology Cell State ImageJ Statistical Analysis Wound Size Cell Proliferation Cell Death Molecular Mechanisms
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