Journal
/
Neuroscience
/
An Injury Paradigm to Investigate Central Nervous System Repair in Drosophila
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
An Injury Paradigm to Investigate Central Nervous System Repair in Drosophila

An Injury Paradigm to Investigate Central Nervous System Repair in Drosophila

DOI:
10.3791/50306-v

•

10:49 min

•

March 28, 2013

•

,
1Neurodevelopment Group, School of Biosciences,University of Birmingham

Chapters

  • 00:05Title
  • 01:18Dissection of Larval VNC
  • 03:56Delivering a Stab Injury to the VNC
  • 06:07Time-lapse Recording of Stabbed VNCs
  • 08:05Post-injury Ventral Nerve Cord Images
  • 10:16Conclusion

Summary

Automatic Translation

Automatic Translation

An injury paradigm using the Drosophila larval ventral nerve cord to investigate central nervous system regeneration and repair is described. Stabbing followed by laser scanning confocal microscopy in time-lapse and fixed specimens, combined with quantitative analysis with purposefully developed software and genetics, are used to investigate the molecular mechanisms of CNS regeneration and repair.

Tags

Keywords: Central Nervous SystemCNSInjuryRepairRegenerationDrosophilaVentral Nerve CordVNCSpinal CordLaser Scanning Confocal MicroscopyImmunofluorescenceImageJCell ProliferationCell DeathGeneticsMolecular Mechanisms

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50306?article_type=v

Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System
check_url/50306?article_type=v

Multimodal Imaging of Stem Cell Implantation in the Central Nervous System of Mice
check_url/50306?article_type=v

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
check_url/50306?article_type=v

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster
check_url/50306?article_type=v

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System
check_url/50306?article_type=v

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
check_url/50306?article_type=v

In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation
check_url/50306?article_type=v

In vivo Optogenetic Stimulation of the Rodent Central Nervous System
check_url/50306?article_type=v

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY
check_url/50306?article_type=v

Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling

Read Article