19,028 Views
•
09:17 min
•
April 24, 2013
DOI:
Summary
Automatically generated
Hier beschreiben wir zwei Tests, die getroffen wurden, um altersabhängige Neurodegeneration dopaminerger (DA) in Neuronen zu studieren<em> Drosophila</em>: Das Klettern / startle-induzierten negativen Geotaxis Assay, um die funktionalen Auswirkungen der DA Neurone Degeneration und der Tyrosinhydroxylase Immunfärbung, die zur Identifizierung und zählen DA Neuronen im gesamten Gehirns Halterungen wird studieren können.
Related Videos
08:24
Dissection and Culture of Mouse Dopaminergic and Striatal Explants in Three-Dimensional Collagen Matrix Assays
Related Videos
25583 Views
16:33
Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)
Related Videos
35326 Views
05:21
Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna
Related Videos
8945 Views
11:58
Primary Culture of Mouse Dopaminergic Neurons
Related Videos
37342 Views
07:27
Olfactory Assays for Mouse Models of Neurodegenerative Disease
Related Videos
21768 Views
08:45
Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains
Related Videos
13818 Views
06:30
Quantitative Analysis of Climbing Defects in a Drosophila Model of Neurodegenerative Disorders
Related Videos
18361 Views
06:47
Assessing Primary Neurogenesis in Xenopus Embryos Using Immunostaining
Related Videos
9450 Views
11:36
Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light
Related Videos
6425 Views
10:36
Electrophysiological Method for Whole-cell Voltage Clamp Recordings from Drosophila Photoreceptors
Related Videos
14861 Views
Read Article
Cite this Article
Barone, M. C., Bohmann, D. Assessing Neurodegenerative Phenotypes in Drosophila Dopaminergic Neurons by Climbing Assays and Whole Brain Immunostaining. J. Vis. Exp. (74), e50339, doi:10.3791/50339 (2013).
Copy