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Culturing Primary Rat Inner Medullary Collecting Duct Cells
JoVE Journal
Biology
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JoVE Journal Biology
Culturing Primary Rat Inner Medullary Collecting Duct Cells
DOI:

06:11 min

June 21, 2013

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Chapters

  • 00:05Title
  • 01:24Preparation
  • 02:01Cultivation of Primary Rat Inner Medullary Collecting Duct (IMCD) Cells
  • 05:09Results: IMCD Cell Expression
  • 05:39Conclusion

Summary

Automatic Translation

Arginine-vasopressin (AVP) controls fine-tuning of body water homeostasis through facilitating water reabsorption by renal principal cells. Here, we present a protocol for the cultivation of primary rat inner medullary collecting duct cells suitable for the elucidation of molecular mechanisms underlying AVP-mediated water reabsorption.

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