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Biology

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A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
 

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination

Article doi: 10.3791/50374
June 25th, 2014

Summary June 25th, 2014

Assaying in vitro β-cell function using isolated mouse islets of Langerhans is an important component in the study of diabetes pathophysiology and therapeutics. While many downstream applications are available, this protocol specifically describes the measurement of intracellular cyclic adenosine monophosphate (cAMP) as an essential parameter determining β-cell function.

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