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Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation
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Neuroscience
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JoVE Journal Neuroscience
Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

DOI:
10.3791/50412-v

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11:48 min

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July 24, 2013

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, , ,
1Institute of Anatomy and Cell Biology,University of Heidelberg , 2Institut de recherches cliniques de Montreal

Chapters

  • 00:05Title
  • 02:06Preparation of DNA and Glass Micropipettes for Injection
  • 02:57Laparotomy
  • 04:33DNA Injection into the Brain Ventricle
  • 05:59Targeting the Hypothalamus for Electroporation
  • 07:17Finishing the Surgery
  • 08:28Analyzing the Results
  • 09:18Results: Hypothalamus Transfection by In utero Electroporation
  • 10:54Conclusion

Summary

Automatic Translation

Automatic Translation

Despite the functional and medical importance of the hypothalamus, in utero genetic manipulation of its development has rarely been attempted. We show a detailed procedure for in utero electroporation into the mouse hypothalamus and show representative results of total and partial (regional) hypothalamic transfection.

Tags

Keywords: Genetic ManipulationMouseDeveloping HypothalamusIn Utero ElectroporationGenetic ModificationMammalian BrainMouse MutantsDeveloping BrainCortexThalamusHypothalamusHypothalamic NeuroepitheliumMammillary BodyElectroporation

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