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JoVE Journal
Biology

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获取荧光时间推移电影的芽殖酵母和分析单细胞动力学移植物
 
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获取荧光时间推移电影的芽殖酵母和分析单细胞动力学移植物

Article DOI: 10.3791/50456-v 17:01 min July 18th, 2013
July 18th, 2013

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Summary

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我们提出了一个简单的协议来获得生长的酵母细胞的荧光显微镜的电影,和一个基于GUI的软件程序包,提取单细胞的时间序列数据。该分析包括自动沿袭和集成的跟踪数据的目视检查和手工curation分组表决的时间分配。

Tags

77期,微生物学,细胞生物学,分子生物学,遗传学,生物物理学,
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