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Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy
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Bioengineering
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JoVE Journal Bioengineering
Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

DOI:
10.3791/50497-v

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08:41 min

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June 27, 2013

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, , ,
1Department of Physics,Worcester Polytechnic Institute, 2Department of Chemical Engineering,Worcester Polytechnic Institute

Chapters

  • 00:05Title
  • 01:10Calibrate the Spring Constant of Cantilever
  • 02:56AFM/Cell Culture Preparation
  • 04:00Collecting Force Curves of Cell Indentation
  • 05:36Results: AFM Measurement of Cell Stiffness
  • 06:59Results: AFM Measurement of Cell Stiffness
  • 08:19Conclusion

Summary

Automatic Translation

Automatic Translation

This paper demonstrates a protocol to characterize the mechanical properties of living cells by means of microindentation using an Atomic Force Microscope (AFM).

Tags

Keywords: Atomic Force MicroscopyCell StiffnessExtracellular MatrixHertz ModelMicro-indentationCell MechanicsCell Physiology

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