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Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo
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Chapters
- 00:05Title
- 00:56Enucleation and Fixation of Postnatal Eyes
- 02:01Dissection of Postnatal Murine Retinas
- 04:59Immuno/isolectin Staining of the Retinal Vasculature
- 07:07Typical Staining of Endothelial Cell and Pericytes
- 08:20Conclusion
The neonatal murine retina provides a well characterized physiological model of angiogenesis, which permits investigations of the roles of different genes or drugs that modulate angiogenesis in an in vivo context. Immunofluorescent staining to accurately visualize the vascular plexus is pivotal to the success of these types of studies.
