A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Cancer Research Center,Sheba Medical Center, 2The Neufeld Cardiac Research Institute,Tel-Aviv University, 3Department of Physiology and Pharmacology,Tel-Aviv University, 4Imaging Unit, Sackler Faculty of Medicine,Tel-Aviv University, 5Biotechnology and Cell Signaling,Ecole Superieure de Biotechnologie Strasbourg, 6Department of Human Molecular Genetics and Biochemistry,Tel-Aviv University
Chapters
- 00:05Title
- 02:07Preparation of Cells for Live Confocal Imaging
- 05:02Examination of Transfection Efficacy
- 06:42Preparation of Fixed Cells for Confocal Imaging
- 09:20Results: PJ-34 Exhibits Cytotoxic Activity on Cells with Multi Centrosomes
- 15:37Conclusion
La actividad citotóxica de la fenantridina PJ-34 en células de cáncer sometidos a la mitosis se documentó en tiempo real por imagen confocal en vivo. 34 PJ-erradicar el cáncer de mama humano MDA-MB-231 células que albergan actividades extra-centrosomas en la mitosis. A diferencia de la mitosis bi-focal normal, las actividades extra-centrosomas no se agruparon en los dos polos del huso en la presencia de PJ-34.
