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Journal
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Immunology and Infection
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遗传操纵<em>Δku80</em>株功能基因组分析<em>弓形虫</em
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Genetic Manipulation in
Δku80
Strains for Functional Genomic Analysis of
Toxoplasma gondii
Please note that all translations are automatically generated.
Click here for the English version.
遗传操纵<em>Δku80</em>株功能基因组分析<em>弓形虫</em
DOI:
10.3791/50598-v
•
09:52 min
•
July 12, 2013
•
Leah M. Rommereim
,
Miryam A. Hortua Triana
,
Alejandra Falla
,
Kiah L. Sanders
,
Rebekah B. Guevara
,
David J. Bzik
,
Barbara A. Fox
1
Department of Microbiology and Immunology
,
The Geisel School of Medicine at Dartmouth
Chapters
00:05
Title
01:58
Preparation of Targeting DNA Plasmid pGOI
02:48
Transfecting T. gondii Parasites
03:43
Selecting Transfected T. gondii Parasites
07:07
Deletion of HXGPRT Marker and C-terminal Tagging
07:48
Results: Deletion of rop18 and Removal of the HXGPRT Selectable Marker from gra2
08:54
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们报道了一种采用I型和II型<em>Δku80</em菌株<em>弓形虫</em>高效的生成有针对性的基因缺失和基因替换为功能基因组学分析。
Tags
Genetic Manipulation
ΔKU80 Strains
Toxoplasma Gondii
Functional Genomic Analysis
Homologous Recombination
Gene Deletion
Gene Replacement
Gene Tagging
HXGPRT Selectable Marker
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