Journal
/
Neuroscience
/
A Dual Tracer PET-MRI Protocol for the Quantitative Measure of Regional Brain Energy Substrates Uptake in the Rat
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
A Dual Tracer PET-MRI Protocol for the Quantitative Measure of Regional Brain Energy Substrates Uptake in the Rat

A Dual Tracer PET-MRI Protocol for the Quantitative Measure of Regional Brain Energy Substrates Uptake in the Rat

DOI:
10.3791/50761-v

•

15:10 min

•

December 28, 2013

•

, , , , , , ,
1Research Center on Aging and Department of Physiology and Biophysics,Université de Sherbrooke, 2Sherbrooke Molecular Imaging Center, Étienne-Le Bel Clinical Research Center,Université de Sherbrooke, 3Department of Computer Science,Université de Sherbrooke, 4Department of Nuclear Medicine and Radiobiology,Université de Sherbrooke

Chapters

  • 00:05Title
  • 02:19Brain Anatomy with MRI
  • 03:19Dual Tracer PET Acquisitions
  • 08:29Quantitative PET Analysis
  • 12:56Results: Dual Tracer PET-MRI
  • 14:00Conclusion

Summary

Automatic Translation

Automatic Translation

Small-animal positron emission tomography enables the assessment of the brain's two main energy substrates: glucose and ketones. In the present method, 11C-acetoacetate and 18F-fluorodeoxyglucose are injected sequentially in each animal, and their uptake is measured quantitatively in specific brain regions determined from the magnetic resonance images.

Tags

Keywords: Dual Tracer PET-MRIBrain Energy SubstratesGlucoseAcetoacetateCerebral Metabolic RateKinetic ModelingImage-derived Input FunctionNeurodegenerative Diseases

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50761?article_type=v

The NeuroStar TMS Device: Conducting the FDA Approved Protocol for Treatment of Depression
check_url/50761?article_type=v

Stereotaxic Surgery for Excitotoxic Lesion of Specific Brain Areas in the Adult Rat
check_url/50761?article_type=v

A Visual Description of the Dissection of the Cerebral Surface Vasculature and Associated Meninges and the Choroid Plexus from Rat Brain
check_url/50761?article_type=v

The Optokinetic Response as a Quantitative Measure of Visual Acuity in Zebrafish
check_url/50761?article_type=v

In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation
check_url/50761?article_type=v

A Simple Way to Measure Alterations in Reward-seeking Behavior Using Drosophila melanogaster
check_url/50761?article_type=v

A Simple One-step Dissection Protocol for Whole-mount Preparation of Adult Drosophila Brains
check_url/50761?article_type=v

Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry
check_url/50761?article_type=v

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System
check_url/50761?article_type=v

In Vivo Single-Molecule Tracking at the Drosophila Presynaptic Motor Nerve Terminal

Read Article