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Journal / Biology / Protokollerne til gennemførelse af en
JoVE Journal
Biology

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JoVE Journal Biology
Protokollerne til gennemførelse af en
Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
Journal (Biology)
Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
Microvolume Protein Concentration Determination using the NanoDrop 2000c Spectrophotometer
Journal (Biology)
Microvolume Protein Concentration Determination using the NanoDrop 2000c Spectrophotometer
 
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Protokollerne til gennemførelse af en

Article DOI: 10.3791/50762 • 16:11 min • September 16th, 2013
September 16th, 2013 •
*1, *2, 3, 4, 2, 4
1Department of Biology, California Institute of Technology, 2Department of Bioengineering, California Institute of Technology, 3Synthetic Biology Center, Department of Bioengineering, Massachusetts Institute of Technology, 4School of Physics and Astronomy, University of Minnesota
* These authors contributed equally

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Denne fem-dages-protokollen skitserer alle trin, udstyr og supplerende software er nødvendig for at skabe og drive en effektiv endogent Escherichia coli baserede TX-TL cellefrit udtryk system fra bunden. Med reagenser, protokollen tager 8 timer eller mindre til opsætning af en reaktions, indsamle og behandle data.

Tags

Cellebiologi Bioengineering Syntetisk biologi kemi Techniques Syntetisk molekylærbiologi kontrol teori TX-TL celle-fri udtryk in vitro transkription-oversættelse celle-fri proteinsyntese syntetisk biologi systembiologi Escherichia coli celleekstrakt biologiske kredsløb biomolekylær breadboard
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