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Journal
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Medicine
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在体内
19女性
MRI对细胞跟踪
JoVE Journal
Medicine
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JoVE Journal
Medicine
In vivo
19
F MRI for Cell Tracking
Please note that all translations are automatically generated.
Click here for the English version.
在体内
19女性
MRI对细胞跟踪
DOI:
10.3791/50802-v
•
10:05 min
•
November 25, 2013
•
Mangala Srinivas
,
Philipp Boehm-Sturm
,
Markus Aswendt
,
Eberhard D. Pracht
3
,
Carl G. Figdor
,
I. Jolanda de Vries
,
Mathias Hoehn
1
Department of Tumor Immunology, Nijmegen Center for Molecular Life Sciences
,
Radboud University Medical Center
,
2
In-Vivo-NMR Laboratory
,
Max Planck Institute for Neurological Research
,
3
German Center for Neurodegenerative Diseases (DZNE)
Chapters
00:05
Title
02:00
Cell Labeling (Standard Protocol with Coincubation)
02:57
Determination of
19
F/cell using
19
F NMR
04:17
Imaging and Mouse Preparation
05:29
B
1
Correction
07:44
Image Processing
08:45
Results:
19
F-labeled Cells Homing to a Draining Lymph Node
09:35
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们描述了一种通用协议,用于在小鼠模型中具有
活体外
标记的细胞用MRI
体内
细胞的跟踪。包含一个典型的协议,用于细胞标记,图像采集处理和量化。
Tags
19F MRI
Cell Tracking
Noninvasive
Quantitative
Murine Immune Cells
Cell Labeling
Imaging Parameters
Image Processing
Article
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