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Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis
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JoVE Journal Neuroscience
Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis

Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis

DOI:
10.3791/50807-v

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11:42 min

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November 20, 2013

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1Laboratory of Neurobiology, National Institute of Neurological Disorders and Stroke,National Institutes of Health

Chapters

  • 00:05Title
  • 01:29Rapid Freezing and Cryosectioning of Cells Cultured on Coverslips
  • 03:33Cryotransfer of Specimens to the Electron Microscope
  • 05:00Freeze Drying and Imaging of Cells and Organelles
  • 06:35Acquisition of Mitochondrial X-ray Spectra
  • 07:44Qualitative and Quantitative Analysis of X-ray Spectra
  • 09:33Results: Mitochondria Sequester Calcium in Response to Glutamate Receptor Activation
  • 11:17Conclusion

Summary

Automatic Translation

Automatic Translation

This paper describes the application of cryoanalytical electron microscopy to the quantitative measurement of total calcium content and distribution at subcellular resolution in physiologically defined biological specimens.

Tags

Keywords: Electron Probe X-ray MicroanalysisEPMAIntracellular Elemental ContentIntramitochondrial CalciumCryofixationCryosectionsEnergy-dispersive X-ray SpectrometryEDXNeurodegenerative DiseasesExcitotoxic InjuryIschemia Resistance

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