Electron Probe Microanalysis
Electron Probe Microanalysis - Related Videos
11:42
Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis
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Cited by 7 •
2013
This paper describes the application of cryoanalytical electron microscopy to the quantitative measurement of total calcium content and distribution at subcellular resolution in physiologically defined biological specimens.
10:53
Scanning-probe Single-electron Capacitance Spectroscopy
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2013
Scanning-probe single-electron capacitance spectroscopy facilitates the study of single-electron motion in localized subsurface regions. A sensitive charge-detection circuit is incorporated into a cryogenic scanning probe microscope to investigate small systems of dopant atoms beneath the surface of semiconductor samples.
07:24
Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis
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Cited by 2 •
2021
We provide a general outline of quantitative microanalysis methods for estimating the site occupancies of impurities and their chemical states by taking advantage of electron-channeling phenomena under incident electron beam-rocking conditions, which reliably extract information from minority species, light elements, oxygen vacancies, and other point/line/planar defects.
14:55
Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis
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Cited by 7 •
2018
We present a protocol to compare the state of minerals in vesicles released by two human bone cell lines: hFOB 1.19 and Saos-2. Their mineralization profiles were analyzed by Alizarin Red-S (AR-S) staining, ultraviolet (UV) light visualization, transmission electron microscopy (TEM) imaging and energy dispersive X-ray microanalysis (EDX).
13:58
Probing C84-embedded Si Substrate Using Scanning Probe Microscopy and Molecular Dynamics
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Cited by 1 •
2016
This paper reports the nanomaterial fabrication of a fullerene Si substrate inspected and verified by nanomeasurements and molecular dynamic simulation.
FAQs
Each element emits X-rays at characteristic energies after irradiation by the focused electron beam. Measuring those energies allows investigators to distinguish which elements occur in the selected microscopic region. The corresponding X-ray intensities provide information about relative abundance, so a chemical map can be related to the local cellular structure.
Beam focusing confines the analysis to a microscopic region rather than averaging chemical signals across an entire specimen. That spatial selectivity helps associate elemental content with particular neurons, synapses, myelin, or other brain structures. The resulting localization is especially useful when chemical differences occur within closely neighboring cellular regions.
Examining calcium, phosphorus, sulfur, and metals together provides a broader chemical profile of a neural region. Their localized presence and relative abundance can support investigations of mineral balance, metal accumulation, and tissue pathology. This combined view helps connect several elemental changes with the cellular structures in which they occur.
The workflow begins with a prepared sample positioned for analysis. A focused electron beam then irradiates a selected microscopic region, causing atoms to emit characteristic X-rays. Researchers interpret the emitted X-ray energies to identify elements and use their intensities to assess relative abundance, producing localized chemical information for comparison with tissue structure.
In neuroscience, EPMA can examine elemental distributions in neurons, synapses, myelin, and broader brain tissue. These measurements support studies of calcium and phosphorus balance, sulfur-containing composition, metal accumulation, and pathology-related chemical changes. Because the method links elemental content to small anatomical regions, it can help localize where such changes occur.
Structural imaging primarily shows the organization or appearance of tissue, whereas EPMA adds localized chemical evidence. Applying both perspectives can relate a visible cellular feature to the elements present in that same microscopic region. This complementary approach strengthens investigations of cellular mechanisms and disease-related tissue changes without treating chemical composition as separate from anatomy.