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在体外试验 ,以评估免疫调节途径对HIV特异性CD4 + T细胞效应功能的影响
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function
DOI:

09:26 min

October 15, 2013

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Chapters

  • 00:05Title
  • 01:27Depletion and Isolation of PBMCs via Ficoll Separation
  • 02:08Antibody Blockade and Antigen-Specific Stimulation of Cells
  • 02:46Collection and Analysis of Supernatant
  • 03:15Collection and Phenotypic Analysis of Cells via Flow Cytometry
  • 04:09Analysis of Cells via qRT-PCR
  • 05:15Adaptation for Intracellular Cytokine Staining at 48 Hours
  • 05:48Adaptation for Sorting Cells
  • 06:55Results: HIV-specific CD4 T Cell Effector Function
  • 09:03Conclusion

Summary

Automatic Translation

我们开发了一种体外测定,调查免疫调节途径中的细胞因子分泌的HIV特异性CD4 + T细胞的调节中的作用。

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