JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
在体外试验
,以评估免疫调节途径对HIV特异性CD4 + T细胞效应功能的影响
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
In Vitro
Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function
Please note that all translations are automatically generated.
Click here for the English version.
在体外试验
,以评估免疫调节途径对HIV特异性CD4 + T细胞效应功能的影响
DOI:
10.3791/50821-v
•
09:26 min
•
October 15, 2013
•
Filippos Porichis*
1
,
Meghan G. Hart*
1
,
Jennifer Zupkosky
,
Lucie Barblu
2
,
Daniel E. Kaufmann
2
1
The Ragon Institute of MGH, MIT and Harvard
,
2
Centre de Recherche du Centre Hospitalier de l’Université de Montréal (CRCHUM)
Chapters
00:05
Title
01:27
Depletion and Isolation of PBMCs via Ficoll Separation
02:08
Antibody Blockade and Antigen-Specific Stimulation of Cells
02:46
Collection and Analysis of Supernatant
03:15
Collection and Phenotypic Analysis of Cells via Flow Cytometry
04:09
Analysis of Cells via qRT-PCR
05:15
Adaptation for Intracellular Cytokine Staining at 48 Hours
05:48
Adaptation for Sorting Cells
06:55
Results: HIV-specific CD4 T Cell Effector Function
09:03
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我们开发了一种
体外
测定,调查免疫调节途径中的细胞因子分泌的HIV特异性CD4 + T细胞的调节中的作用。
Tags
In Vitro Assay
HIV
CD4 T Cells
Effector Function
Immunoregulatory Pathways
PD-1
IL-10
T Cell Exhaustion
Cytokine Production
PBMC
Flow Cytometry
QRT-PCR
Gag Peptide Pool
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
T细胞受体在小鼠T细胞的逆转录病毒转导
隔离和幼稚的CD4 T细胞的Th17细胞分化
使用一个调查益生素对肺炎球菌定植的影响<em>体外</em>粘附试验
尾部皮肤来研究异基因CD4 + T细胞应答的小鼠移植
基于图像的流式细胞仪技术来评估变化粒细胞功能<em>在体外</em
诱导小鼠肠道炎症由效应CD4的过继转移<sup> +</sup> CD45RB<sup>高</sup> T细胞免疫缺陷小鼠
鼠标朴素CD4<sup> +</sup> T细胞分离和<em>在体外</em>分化为T细胞亚群
长期培养的干扰素γ酶联免疫检测方法的评估效应和记忆T细胞应答的牛应用
成像的CD4 T细胞间质迁移发炎真皮
一个<em>体外</em>血 - 脑屏障使用阻抗谱型号:对T细胞,内皮细胞的相互作用为中心
Read Article