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JoVE Journal
Neuroscience

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Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro
 

Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro

Article doi: 10.3791/50989
November 12th, 2013 Usage Statistics

Summary November 12th, 2013

Neuroblast migration is a crucial step in postnatal neurogenesis. The protocol described here can be used to investigate the role of candidate regulators of neuroblast migration by employing DNA/small hairpin RNA (shRNA) nucleofection and a 3D migration assay with neuroblasts isolated from the rodent postnatal rostral migratory stream.

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