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Long Term Chronic Pseudomonas aeruginosa Airway Infection in Mice
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Immunology and Infection
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JoVE Journal Immunology and Infection
Long Term Chronic Pseudomonas aeruginosa Airway Infection in Mice

Long Term Chronic Pseudomonas aeruginosa Airway Infection in Mice

DOI:
10.3791/51019-v

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15:43 min

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March 17, 2014

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, , ,
1Infections and Cystic Fibrosis Unit, Division of Immunology, Transplantation and Infectious Diseases,San Raffaele Scientific Institute, 2Italian Cystic Fibrosis Research Foundation

Chapters

  • 00:05Title
  • 03:04Bacteria Preparation in Agar Beads for Chronic Infection
  • 06:36Mice Challenge with Agar-beads
  • 07:58Bronchoalveolar Lavage Fluid Collection and Analysis
  • 11:06Results: Long-term Chronic Pseudomonas aeruginosa Airway Infection in Mice
  • 13:43Conclusion

Summary

Automatic Translation

Automatic Translation

We describe the agar-beads method to establish persistent long-term chronic Pseudomonas aeruginosa airway infection in the mouse model.

Tags

Keywords: Chronic Pseudomonas AeruginosaAirway InfectionMouse ModelCystic FibrosisAgar-beadsP. Aeruginosa RP73 StrainLong-term InfectionLung PathologyInflammatory Response

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