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JoVE Journal Neuroscience
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Isolation and Culture of Endothelial Cells from the Embryonic Forebrain

Article DOI: 10.3791/51021-v • 12:02 min • January 23rd, 2014
January 23rd, 2014 •
1,2, 1,2
1Department of Psychiatry, Harvard Medical School, 2Angiogenesis and Brain Development Laboratory, Division of Basic Neuroscience, McLean Hospital

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Summary
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This video demonstrates an easy and reliable strategy for preparation of pure cultures of endothelial cells from the embryonic forebrain within 10-12 days and will be useful for research focused on many aspects of cerebral angiogenesis.

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Isolation Culture Endothelial Cells Embryonic Forebrain Angiogenesis Neurovascular Development Pial Vascular Network Periventricular Vascular Network Gene Expression Profiles Verification Pure Populations Protocol Telencephalon Pial Membrane Collagenase/dispase Digestion Single Cell Suspension Positive Selection Anti-CD-31/PECAM-1 Antibody MicroBeads Magnetic Separation Method Collagen 1 Coating Cell Culture Medium Confluent Subculture Cobblestone Phenotype Spindle Phenotype Phase-contrast Light Microscopy Fluorescence Microscopy Purity Assessment
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