A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

Christophe. J Queval; Ok-Ryul Song; Vincent Delorme; Raffaella Iantomasi; Romain Veyron-Churlet; Nathalie Deboos&#232;re; Val&#233;rie Landry; Alain Baulard; Priscille Brodin

doi:10.3791/51114

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A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

7,669 Views

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15:28 min

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January 17, 2014

DOI:

10.3791/51114-v

DOI:

10.3791/51114-v

•

15:28 min

January 17, 2014

•

4 Views

Christophe. J Queval*¹, Ok-Ryul Song*¹, Vincent Delorme*¹, Raffaella Iantomasi¹, Romain Veyron-Churlet¹, Nathalie Deboosère¹, Valérie Landry¹, Alain Baulard¹, Priscille Brodin¹

¹Inserm U1019 – CNRS UMR 8024, Institut Pasteur de Lille,Université de Lille

Summary

Here, we describe a phenotypic assay applicable to the High-throughput/High-content screens of small-interfering synthetic RNA (siRNA), chemical compound, and Mycobacterium tuberculosis mutant libraries. This method relies on the detection of fluorescently labeled Mycobacterium tuberculosis within fluorescently labeled host cell using automated confocal microscopy.