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Immunology and Infection

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JoVE Journal Immunology and Infection
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A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

Article DOI: 10.3791/51114
January 17th, 2014 •
*1, *1, *1, 1, 1, 1, 1, 1, 1
1Inserm U1019 - CNRS UMR 8024, Institut Pasteur de Lille, Université de Lille
* These authors contributed equally

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January 17th, 2014

Here, we describe a phenotypic assay applicable to the High-throughput/High-content screens of small-interfering synthetic RNA (siRNA), chemical compound, and Mycobacterium tuberculosis mutant libraries. This method relies on the detection of fluorescently labeled Mycobacterium tuberculosis within fluorescently labeled host cell using automated confocal microscopy.

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