Journal
/
Immunology and Infection
/
Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

DOI:
10.3791/51151-v

•

09:27 min

•

January 17, 2014

•

, , , , ,
1School of Molecular Bioscience,University of Sydney, 2Department of Medicine,Center for Vascular Research, 3Asia Pacific Centre for Animal Health, Faculty of Veterinary Science,University of Melbourne

Chapters

  • 00:05Title
  • 01:34Creating the Recombinant Vector
  • 02:48Recombinant Virus Generation
  • 05:26Plaque Assay: NO Selection
  • 05:59Purity of Viral Stocks: PCR Screening of Genomic DNA
  • 07:23Results: Recombinant Vectors and the VACV Genome
  • 08:43Conclusion

Summary

Automatic Translation

Automatic Translation

A rapid and modular protocol for the generation of recombinant vaccinia viruses expressing fluorescently tagged proteins simultaneously using the method of transient dominant selection is described here.

Tags

Keywords: Fluorescent Protein TaggingVaccinia VirusRecombinant Virus GenerationHomologous RecombinationTransient Dominant SelectionMetabolic SelectionLive-cell MicroscopyVirus-host Interactions

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/51151?article_type=v

Generation of Recombinant Influenza Virus from Plasmid DNA
check_url/51151?article_type=v

RNAi Screening for Host Factors Involved in Vaccinia Virus Infection using Drosophila Cells
check_url/51151?article_type=v

Finger-stick Blood Sampling Methodology for the Determination of Exercise-induced Lymphocyte Apoptosis
check_url/51151?article_type=v

A Functional Whole Blood Assay to Measure Viability of Mycobacteria, using Reporter-Gene Tagged BCG or M.Tb (BCG lux/M.Tb lux)
check_url/51151?article_type=v

Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System
check_url/51151?article_type=v

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection
check_url/51151?article_type=v

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
check_url/51151?article_type=v

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
check_url/51151?article_type=v

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy
check_url/51151?article_type=v

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus

Read Article