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Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

DOI:
10.3791/51177-v

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16:21 min

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March 10, 2014

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, ,
1Microbiology Unit, Department of Biochemistry,University of Oxford, 2Biological Physics Research Group, Clarendon Laboratory, Department of Physics,University of Oxford

Chapters

  • 00:05Title
  • 01:32Cell Slide Preparation
  • 04:00Preparing Microscopy Data Acquisition
  • 06:43Acquiring Photo-activated Localization Microscopy (PALM) Movies
  • 08:16PALM Movie Analysis
  • 12:48Representative Results: Pol1-DNA Binding in Single Bacterial Cells
  • 15:53Conclusion

Summary

Automatic Translation

Automatic Translation

Photoactivated localization microscopy (PALM) combined with single-molecule tracking allows direct observation and quantification of protein-DNA interactions in live Escherichia coli cells.

Tags

Keywords: Protein-DNA InteractionsLive Bacterial CellsPhotoactivated Single-molecule TrackingDNA ReplicationTranscriptionRepairChromosome OrganizationDNA-binding ProteinsPhotoactivated Localization Microscopy (PALM)Single-molecule TrackingDNA-binding EventsProtein ActivitySingle-cell Level

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