Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress

Olivier Etienne; Amandine Bery; Telma Roque; Chantal Desmaze; Fran&#231;ois D. Boussin

doi:10.3791/51209

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Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress

15,216 Views

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09:51 min

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May 07, 2014

DOI:

10.3791/51209-v

DOI:

10.3791/51209-v

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09:51 min

May 07, 2014

•

2 Views

Olivier Etienne^1,2,3,4, Amandine Bery^1,2,3,4, Telma Roque^1,2,3,4, Chantal Desmaze^1,2,3,4, François D. Boussin^1,2,3,4

¹Laboratoire de Radiopathologie,CEA DSV iRCM SCSR, ²INSERM, U967, ³Université Paris Diderot, Sorbonne Paris Cité, ⁴Université Paris Sud, UMR 967

Summary

Administration of two analogs of thymidine, EdU and BrdU, in pregnant mice allows the analysis of cell cycle progression in neural and progenitor cells in the embryonic mouse brain. This method is useful to determine the effects of genotoxic stress, including ionizing radiation, during brain development.