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In vitro Method to Observe E-selectin-mediated Interactions Between Prostate Circulating Tumor Cells Derived From Patients and Human Endothelial Cells
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JoVE Journal Medicine
In vitro Method to Observe E-selectin-mediated Interactions Between Prostate Circulating Tumor Cells Derived From Patients and Human Endothelial Cells

In vitro Method to Observe E-selectin-mediated Interactions Between Prostate Circulating Tumor Cells Derived From Patients and Human Endothelial Cells

DOI:
10.3791/51468-v

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11:56 min

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May 15, 2014

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, ,
1Department of Medicine,Weill Cornell Medical College, 2Department of Urology,Weill Cornell Medical College

Chapters

  • 00:05Title
  • 01:50Culturing HUVECs on Microslides for Observing Circulating Tumor Cell (CTC)-endothelial Interactions
  • 04:04Preparation of Flow Chamber Assembly for Overnight HUVEC Culture on a Microslide
  • 05:48Preparation of Anti-PSMA Labeled Prostate Cancer Cells
  • 07:05Preparation of Microscope, Syringe Pump and Flow Chamber Assembly
  • 09:11Results: Analysis of Interactions Between Labeled MDA Prostate Cancer Cells and Endothelial Cells
  • 11:11Conclusion

Summary

Automatic Translation

Automatic Translation

Our report describes a unique method to visualize and analyze CTC/EC interactions in prostate cancer under physiological flow conditions.

Tags

Keywords: In VitroE-selectinProstate Circulating Tumor CellsHuman Endothelial CellsMetastasisFlow ChamberCirculating Tumor CellsProstate Cancer CellsCell Interactions

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