Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Yang Song; Xuemei Zhang; Lingyan Huang; Mark A. Behlke; Andrew Tsourkas

doi:10.3791/51544

Journal

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Biology

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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

JoVE Journal
Biology

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JoVE Journal Biology

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

11,734 Views

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12:20 min

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August 06, 2014

DOI:

10.3791/51544-v

DOI:

10.3791/51544-v

•

12:20 min

August 06, 2014

•

2 Views

Yang Song*¹, Xuemei Zhang*¹, Lingyan Huang², Mark A. Behlke², Andrew Tsourkas¹

¹Department of Bioengineering,University of Pennsylvania, ²Integrated DNA Technologies, Inc.

Summary

Ratiometric bimolecular beacons (RBMBs) can be used to image single engineered RNA transcripts in living cells. Here, we describe the preparation and purification of RBMBs, delivery of RBMBs into cells by microporation and fluorescent imaging of single RNA transcripts in real-time.