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革兰从血培养液使用MALDI-TOF质谱阴性细菌快速鉴定
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry
DOI:

13:29 min

May 28, 2014

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Chapters

  • 00:05Title
  • 01:20Preparation of Bacterial Inoculate from Blood Culture Broth
  • 05:46Preparation of MALDI-TOF Target Plate
  • 06:34MALDI-TOF MS Spectra Acquisition
  • 08:42Post Analysis-Interpretation of MALDI-TOF MS Scores
  • 10:18Representative MALDI-TOF Analysis of Gram Negative Bacteria from Blood Cultures
  • 12:35Conclusion

Summary

Automatic Translation

的飞行(MALDI-TOF)质谱(MS),直接向血培养肉汤基质辅助激光解吸/电离飞行时间质谱的应用加快细菌的鉴定。该方法是一种快速和可靠的方法,可直接从血培养肉汤鉴定革兰氏阴性细菌。

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