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Journal
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Immunology and Infection
/
革兰从血培养液使用MALDI-TOF质谱阴性细菌快速鉴定
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry
Please note that all translations are automatically generated.
Click here for the English version.
革兰从血培养液使用MALDI-TOF质谱阴性细菌快速鉴定
DOI:
10.3791/51663-v
•
13:29 min
•
May 28, 2014
•
Timothy J. Gray
,
Lee Thomas
,
Tom Olma
,
David H. Mitchell
,
Jon R. Iredell
2,3
,
Sharon C. A. Chen
3
1
Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research
,
Westmead Hospital
,
2
Centre for Research Excellence in Critical Infection, Westmead Millennium Institute
,
Westmead Hospital
,
3
Sydney Emerging Infectious Diseases Institute, University of Sydney
,
Westmead Hospital
Chapters
00:05
Title
01:20
Preparation of Bacterial Inoculate from Blood Culture Broth
05:46
Preparation of MALDI-TOF Target Plate
06:34
MALDI-TOF MS Spectra Acquisition
08:42
Post Analysis-Interpretation of MALDI-TOF MS Scores
10:18
Representative MALDI-TOF Analysis of Gram Negative Bacteria from Blood Cultures
12:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
的飞行(MALDI-TOF)质谱(MS),直接向血培养肉汤基质辅助激光解吸/电离飞行时间质谱的应用加快细菌的鉴定。该方法是一种快速和可靠的方法,可直接从血培养肉汤鉴定革兰氏阴性细菌。
Tags
Rapid Identification
Gram Negative Bacteria
Blood Culture Broth
MALDI-TOF Mass Spectrometry
Clinical Microbiology
Bacterial Identification
Bloodstream Infection
Sub-culture
Solid Agar
MALDI-TOF MS
Blood Culture Specimens
Interfering Resins
Proteins
Hemoglobin
Bacterial Concentration
Bacterial Purification
Bacterial Extraction
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