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In vivo Klonale Tracking van hematopoietische stamcellen en progenitorcellen Gekenmerkt door Five fluorescerende eiwitten met behulp van confocale microscopie en Multiphoton

Article DOI: 10.3791/51669-v • 17:08 min • August 6th, 2014
August 6th, 2014 •
1, 2, 2
1Light Microscopy Core Facility, NHLBI/NIH, 2Hematology Branch, NHLBI/NIH

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Combinatorische 5 fluorescerende eiwitten markering van hematopoietische stamcellen en voorlopercellen maakt in vivo klonale volgen via confocale en twee-foton microscopie, het verstrekken van inzicht in het beenmerg hematopoietische architectuur tijdens de regeneratie. Deze methode maakt het mogelijk niet-invasieve lot in kaart brengen van spectraal-gecodeerde HSPCs-afgeleide cellen in intacte weefsels voor langere perioden na de transplantatie.

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Stem Cell Biology LEGO beeldvorming klonale tracking fluorescerende eiwitten confocale microscopie multifoton microscopie hematopoiesis lentivirale vectoren hematopoietische stamcellen
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