Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.

JoVE Journal Biology
Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
Isolation of Microvascular Endothelial Tubes from Mouse Resistance Arteries
Journal (Biology)
Isolation of Microvascular Endothelial Tubes from Mouse Resistance Arteries
Long-term, High-resolution Confocal Time Lapse Imaging of Arabidopsis Cotyledon Epidermis during Germination
Journal (Biology)
Long-term, High-resolution Confocal Time Lapse Imaging of Arabidopsis Cotyledon Epidermis during Germination
 

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes

Article DOI: 10.3791/51720
October 23rd, 2014 •
1,2, 1, 1,2, 1,2
1Stowers Institute for Medical Research, 2Department of Biochemistry & Molecular Biology, Kansas University Medical Center

Chapters

Summary
Automatic Translation
October 23rd, 2014

This protocol describes a procedure for generating and purifying wild type and mutant versions of the human INO80 chromatin remodeling complex. Epitope tagged versions of INO80 subunits are stably expressed in HEK293 cells, and complete complexes and complexes lacking specific sets of subunits are purified by immunoaffinity chromatography.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter