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JoVE Journal
Bioengineering

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Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
 

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Article DOI: 10.3791/51762
October 17th, 2014

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Summary October 17th, 2014

Here we present a simple, rapid method for characterizing the intrinsic adhesive properties of putative cell adhesion molecules. The secreted, epitope-tagged ectodomain of a cell adhesion molecule is captured from the culture medium on small, uniform functionalized beads. These beads can then be used immediately in simple bead aggregation assays.

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