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Zinkfinger-Nuklease Verbesserte Gene Targeting in humanen embryonalen Stammzellen
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Zinkfinger-Nuklease Verbesserte Gene Targeting in humanen embryonalen Stammzellen

Article DOI: 10.3791/51764-v • 12:13 min • August 23rd, 2014
August 23rd, 2014 •
1, 1, 1, 1, 1
1Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University

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Reporterzelllinien bieten ein Mittel, um zu visualisieren, zu verfolgen und zu isolieren Zellen von Interesse aus heterogenen Populationen. Jedoch Gen-Targeting unter Verwendung von herkömmlichen homologen Rekombination in embryonalen Stammzellen äußerst ineffizient. Hier beschreiben wir Targeting ZNS Mittelhirn spezifischen Transkriptionsfaktor PITX3 Ortes mit EGFP mit Zink-Finger-Nuklease verbesserte homologe Rekombination.

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Molecular Biology Ausgabe 90 Elektroporation mit menschlichen embryonalen Stammzellen Genom Bearbeitung Reporterzelllinie dopaminergen Mittelhirnneuronen
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