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Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination
Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination

Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination

18,355 Views

09:45 min

August 25, 2014

DOI:

09:45 min
August 25, 2014

18318 Views
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Summary

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A technique to study NG2 cells and oligodendrocytes using a slice culture system of the forebrain and cerebellum is described. This method allows examination of the dynamics of proliferation and differentiation of cells within the oligodendrocyte lineage where the extracellular environment can be easily manipulated while maintaining tissue cytoarchitecture.

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