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JoVE Journal
Neuroscience

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Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination
 

Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination

Article DOI: 10.3791/51835
August 25th, 2014

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Summary August 25th, 2014

A technique to study NG2 cells and oligodendrocytes using a slice culture system of the forebrain and cerebellum is described. This method allows examination of the dynamics of proliferation and differentiation of cells within the oligodendrocyte lineage where the extracellular environment can be easily manipulated while maintaining tissue cytoarchitecture.

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