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Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination
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JoVE Journal Neuroscience
Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination

Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination

DOI:
10.3791/51835-v

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09:45 min

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August 25, 2014

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, , ,
1Department of Physiology and Neurobiology,University of Connecticut, 2Stem Cell Institute,University of Connecticut, 3Department of Neurology,Yale University School of Medicine

Chapters

  • 00:05Title
  • 01:24Preparing Tissue Slices
  • 04:10Time-Lapse Imaging of NG2 Cell Division
  • 05:39Slice Immunohistochemistry
  • 07:17NG2 Cell Differentiation and Mature Oligodendrocyte Characterization
  • 09:08Conclusion

Summary

Automatic Translation

Automatic Translation

A technique to study NG2 cells and oligodendrocytes using a slice culture system of the forebrain and cerebellum is described. This method allows examination of the dynamics of proliferation and differentiation of cells within the oligodendrocyte lineage where the extracellular environment can be easily manipulated while maintaining tissue cytoarchitecture.

Tags

Keywords: Organotypic Slice CulturesOligodendrocyte DynamicsMyelinationNG2 CellsPolydendrocytesOligodendrocyte Precursor CellsPrimary Dissociated CulturesNeuron CoculturesTransgenic Mouse LinesForebrainCerebellumCellular BehaviorCellular InteractionsRegion-dependent Heterogeneity

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