Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders

Soumya Narayan; Charlee McLean; Akira Sawa; Sandra Y. Lin; Narayan Rai; MariaMananita S. Hipolito; Nicola Cascella; John J.I. Nurnberger, Jr.; Koko Ishizuka; Evaristus A. Nwulia

doi:10.3791/51853

Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Po...

JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Neuroscience

Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders

Full Text

10,207 Views

08:33 min

December 4, 2014

DOI: 10.3791/51853-v

Soumya Narayan¹, Charlee McLean², Akira Sawa¹, Sandra Y. Lin³, Narayan Rai², MariaMananita S. Hipolito², Nicola Cascella⁴, John J.I. Nurnberger, Jr.⁵, Koko Ishizuka¹, Evaristus A. Nwulia²

¹Department of Psychiatry,Johns Hopkins University, ²Department of Psychiatry and Behavioral Sciences,Howard University, ³Department of Otolaryngology-Head and Neck Surgery,Johns Hopkins University, ⁴Department of Psychiatry,Sheppard Pratt Hospital, ⁵Department of Psychiatry,Indiana University

In this study, a novel platform to investigate intraneuronal molecular signatures of treatment response in bipolar disorder (BD) was developed and validated. Olfactory epithelium from BD patients was obtained through nasal biopsies. Then laser-capture microdissection was combined with Real Time RT PCR to investigate the molecular signature of lithium response in BD.

This procedure details the use of laser capture micro dissection to isolate neuronal layers from the olfactory epithelium tissue, and also outlines some downstream gene expression analyses. Start with a nasal biopsy to obtain the olfactory epithelium tissue. Then perform laser capture microdissection to isolate the neuronal layers.

Next isolate RNA from the samples. Reverse transcribe for CDNA and analyze for gene expression by QPCR. Ultimately, laser capture microdissection is used to show that an enriched population of neurons can be isolated from olfactory epithelium tissue and applied to numerous downstream applications.

View the full transcript and gain access to thousands of scientific videos