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Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction
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Neuroscience
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JoVE Journal Neuroscience
Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction

Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction

DOI:
10.3791/51863-v

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10:15 min

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September 15, 2014

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, , , ,
1Department of Neuroscience,Baylor College of Medicine, 2Department of Neuroscience, Center for Translational Research in Neurodegenerative Disease,University of Florida, 3Department of Neurology and Department of Neurosurgery,Baylor College of Medicine

Chapters

  • 00:05Title
  • 01:24Staging the Newborn Pups for Injection
  • 02:38Free-hand Intracranial Injection of AAV into Neonatal Mice
  • 04:54Stereotaxic Injection of AAV into Neonatal Mice
  • 07:21Post-injection Care
  • 07:54Effect of Viral Titer and Serotype on VIral Transduction and Co-expression
  • 09:55Conclusion

Summary

Automatic Translation

Automatic Translation

Here we demonstrate a technique for widespread neuronal transduction by intraventricular injection of adeno-associated virus into the neonatal mouse brain. This method provides a rapid and easy way to attain lifelong expression of virally-delivered transgenes.

Tags

IntracerebroventricularViral InjectionNeonatal Mouse BrainNeuronal TransductionAdeno-associated VirusAAVEpendymal LiningGene ExpressionFluorescent ProteinViral TiterStereotaxic Frame

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