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JoVE Journal
Biology

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Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells
 

Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach

Article DOI: 10.3791/51897
October 16th, 2014

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Summary October 16th, 2014

The expression of recombinant proteins by mammalian systems is becoming an attractive method for producing protein complexes for structural biology. Here we present a simple yet highly efficient expression system using suspension grown mammalian cells to purify protein complexes for structural studies.

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