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gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair
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gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

DOI:
10.3791/51902-v

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08:15 min

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October 06, 2014

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1Department of Biology and Pathology of Tumors, Unit of Molecular Biology, Platform of Immunomonitoring and Genetics,Centre Georges-François Leclerc

Chapters

  • 00:05Title
  • 01:14gDNA Enrichment: Day 1, Morning
  • 03:11gDNA Enrichment: Day 1, Afternoon
  • 03:50gDNA Enrichment: Day 2, Morning
  • 06:16gDNA Enrichment: Day 2, Afternoon and Day 3
  • 07:19Results: gDNA Enrichment by Transposase-based Technology
  • 07:55Conclusion

Summary

Automatic Translation

Automatic Translation

gDNA enrichment for NGS sequencing is an easy and powerful tool for the study of constitutional mutations. In this article, we present the procedure to analyse simply the complete sequence of 11 genes involved in DNA damage repair.

Tags

Keywords: NGSNext-generation SequencingCancer GeneticsDNA Damage RepairBRCA1BRCA2Transposase-based TechnologyGDNA EnrichmentGenetic DiagnosisSample Multiplexing

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