Measuring Spatial and Temporal Ca<sup>2+</sup> Signals in Arabidopsis Plants

Xiaohong Zhu; Aaron Taylor; Shenyu Zhang; Dayong Zhang; Ying Feng; Gaimei Liang; Jian-Kang Zhu

doi:10.3791/51945

Journal

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Biology

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Measuring Spatial and Temporal Ca²⁺ Signals in Arabidopsis Plants

Measuring Spatial and Temporal Ca<sup>2+</sup> Signals in Arabidopsis Plants

JoVE Journal
Biology

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JoVE Journal Biology

Measuring Spatial and Temporal Ca²⁺ Signals in Arabidopsis Plants

12,045 Views

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10:12 min

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September 02, 2014

DOI:

10.3791/51945-v

DOI:

10.3791/51945-v

•

10:12 min

September 02, 2014

•

12029 Views

Xiaohong Zhu¹, Aaron Taylor², Shenyu Zhang¹, Dayong Zhang^1,3, Ying Feng^1,4, Gaimei Liang^1,5, Jian-Kang Zhu^1,6

¹Department of Horticulture and Landscape Architecture,Purdue University ²Bindley Bioscience Center,Purdue University ³Institute of Biotechnology,Jiangsu Academy of Agricultural Sciences ⁴College of Environmental & Resource Science,Zhejiang University ⁵Dryland Agriculture Research Centre,Shanxi Academy of Agricultural Sciences ⁶Shanghai Center for Plant Stress Biology,Chinese Academy of Sciences

Summary

Ca²⁺ signaling regulates diverse biological processes in plants. Here we present approaches for monitoring abiotic stress induced spatial and temporal Ca²⁺ signals in Arabidopsis cells and tissues using the genetically encoded Ca²⁺ indicators Aequorin or Case12.