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Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants
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Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants

Article DOI: 10.3791/51945 • 10:12 min • September 2nd, 2014
September 2nd, 2014 •
1, 2, 1, 1,3, 1,4, 1,5, 1,6
1Department of Horticulture and Landscape Architecture, Purdue University, 2Bindley Bioscience Center, Purdue University, 3Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, 4College of Environmental & Resource Science, Zhejiang University, 5Dryland Agriculture Research Centre, Shanxi Academy of Agricultural Sciences, 6Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences

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Summary
Automatic Translation

Ca2+ signaling regulates diverse biological processes in plants. Here we present approaches for monitoring abiotic stress induced spatial and temporal Ca2+ signals in Arabidopsis cells and tissues using the genetically encoded Ca2+ indicators Aequorin or Case12.

Tags

Spatial Temporal Ca2+ Signals Arabidopsis Plants Developmental Cues Environmental Cues Ca2+ Fluctuations Stimulus-specific Cellular Ca2+ Binding Proteins Ca2+ Signaling Cascades Ca2+ Channels Ca2+ Transporters Cellular Components Tissue Level Cellular Level Genetically Encoded Ca2+ Indicators Live Cell Confocal Imaging Aequorin Based FAS Luminescence Ca2+ Imaging Case12 Based Live Cell Confocal Fluorescence Ca2+ Imaging
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