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Neuroscience

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JoVE Journal Neuroscience
Isolation and Culture of Dissociated Sensory Neurons From Chick Embryos
Whole Cell Recording from an Organotypic Slice Preparation of Neocortex
Journal (Neuroscience)
Whole Cell Recording from an Organotypic Slice Preparation of Neocortex
Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
Journal (Neuroscience)
Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
 

Isolation and Culture of Dissociated Sensory Neurons From Chick Embryos

Article DOI: 10.3791/51991
September 24th, 2014 •
1, 1, 1
1Department of Natural Sciences, Assumption College

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September 24th, 2014

Cell culture models provide detailed control over environmental conditions and thus provide a powerful platform to elucidate numerous aspects of neuronal cell biology. We describe a rapid, inexpensive, and reliable method to isolate, dissociate, and culture sensory neurons from chick embryos. Details of substrata preparation and immunocytochemistry are also provided.

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