Journal
/
Neuroscience
/
Isolation and Culture of Dissociated Sensory Neurons From Chick Embryos
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Isolation and Culture of Dissociated Sensory Neurons From Chick Embryos

Isolation and Culture of Dissociated Sensory Neurons From Chick Embryos

DOI:
10.3791/51991-v

•

11:16 min

•

September 24, 2014

•

, ,
1Department of Natural Sciences,Assumption College

Chapters

  • 00:05Title
  • 01:48Chick Dissection and Isolation of DRGs
  • 04:54Dissociation, Enriching, and Culturing DRG Neurons – Part 1
  • 07:29Dissociation, Enriching, and Culturing DRG Neurons – Part 2
  • 09:09Results: Isolation and Culture of Dissociated Sensory Neurons from Chick Embryos
  • 10:12Conclusion

Summary

Automatic Translation

Automatic Translation

Cell culture models provide detailed control over environmental conditions and thus provide a powerful platform to elucidate numerous aspects of neuronal cell biology. We describe a rapid, inexpensive, and reliable method to isolate, dissociate, and culture sensory neurons from chick embryos. Details of substrata preparation and immunocytochemistry are also provided.

Tags

Keywords: Neuron IsolationCell CultureChick EmbryoSensory NeuronsPrimary NeuronsLaminin SubstrateIn Vitro ModelImmunocytochemistryLive Cell ImagingRNAi

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/51991?article_type=v

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord
check_url/51991?article_type=v

The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds
check_url/51991?article_type=v

Live-imaging of PKC Translocation in Sf9 Cells and in Aplysia Sensory Neurons
check_url/51991?article_type=v

Isolation and Culture of Hippocampal Neurons from Prenatal Mice
check_url/51991?article_type=v

Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents
check_url/51991?article_type=v

Production and Isolation of Axons from Sensory Neurons for Biochemical Analysis Using Porous Filters
check_url/51991?article_type=v

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains
check_url/51991?article_type=v

Stripe Assay to Study the Attractive or Repulsive Activity of a Protein Substrate Using Dissociated Hippocampal Neurons
check_url/51991?article_type=v

Removal of Drosophila Muscle Tissue from Larval Fillets for Immunofluorescence Analysis of Sensory Neurons and Epidermal Cells
check_url/51991?article_type=v

Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons

Read Article