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Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures
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Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures

Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures

DOI:
10.3791/52026-v

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08:53 min

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November 03, 2014

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,
1Department of Medicine,Albert Einstein College of Medicine, 2Department of Cell Biology,Albert Einstein College of Medicine

Chapters

  • 00:05Title
  • 01:42Crypt Isolation and Culture
  • 06:17Extracellular Flux Assay
  • 07:36Results: Metabolism Assay Using Organoids Derived from 8 month old C57BL/6 Mice
  • 08:20Conclusion

Summary

Automatic Translation

Automatic Translation

Small intestinal crypt organoids cultured ex vivo provide a tissue culture system that recapitulates growth of crypts dependent on stem cells and their niche. We established a method to assay the metabolic profile in real time in primary mouse crypt organoids. We found organoids maintain physiological properties defined by their source.

Tags

Keywords: Intestinal Crypt OrganoidMouse IntestineMetabolic ProfileOxygen ConsumptionGlycolytic RateATP ProductionRespiratory CapacityReal-time AnalysisCrypt MicroenvironmentIntestinal Epithelial CellsAdult Stem CellsProgenitor CellsPaneth CellsStromal CellsImmune Cells

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