An <em>Ex Vivo</em> Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

Starlyn L. M. Okada; Nicole S. Stivers; Peter K. Stys; David P. Stirling

doi:10.3791/52173

Journal

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Neuroscience

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An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

An <em>Ex Vivo</em> Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

10,811 Views

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11:18 min

•

November 25, 2014

DOI:

10.3791/52173-v

DOI:

10.3791/52173-v

•

11:18 min

November 25, 2014

•

1 Views

Starlyn L. M. Okada¹, Nicole S. Stivers¹, Peter K. Stys², David P. Stirling¹

¹KY Spinal Cord Injury Research Center, Department of Neurological Surgery,University of Louisville, ²Hotchkiss Brain Institute, Department of Clinical Neurosciences,University of Calgary

Summary

We present a protocol utilizing two-photon excitation time-lapse microscopy to simultaneously visualize the dynamics of axon and myelin injuries in real time. This proposed protocol permits studies of both intrinsic and extrinsic factors which can influence central myelinated axon fate after injury and contribute to permanent clinical disability.