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An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time
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JoVE Journal Neuroscience
An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

DOI:
10.3791/52173-v

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11:18 min

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November 25, 2014

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, , ,
1KY Spinal Cord Injury Research Center, Department of Neurological Surgery,University of Louisville, 2Hotchkiss Brain Institute, Department of Clinical Neurosciences,University of Calgary

Chapters

  • 00:05Title
  • 01:43Preparations
  • 03:15Dissection of Adult Murine Cervical Spinal Cord
  • 06:36Nile Red Labeling and Imaging with Laser–induced Injury
  • 09:01Results: Dynamic Injury Response Recordings
  • 10:31Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol utilizing two-photon excitation time-lapse microscopy to simultaneously visualize the dynamics of axon and myelin injuries in real time. This proposed protocol permits studies of both intrinsic and extrinsic factors which can influence central myelinated axon fate after injury and contribute to permanent clinical disability.

Tags

Axonal DegenerationSpinal Cord InjuryEx Vivo ModelTwo-photon MicroscopyAxonal RetractionMyelin DegenerationAxonal SwellingAxonal TransectionReal-time Imaging

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