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Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models
This content is Free Access.
Chapters
- 00:05Title
- 01:28Three-dimensional Cell Culture and Preparation of Agarose Coated 96 Well Plates
- 02:32Seed and Harvest the Three-dimensional Cultures and Embed in Gelatin
- 04:11Slice and Thaw Mount the Gelatin-embedded Three-dimensional Cell Cultures for Mass Spectrometric Imaging
- 05:03Matrix Application and Sample Preparation for MALDI Imaging
- 05:42MALDI-MSI Analysis Using a MALDI-TOF System and Optional Data Analysis Methods
- 07:28Results: Mass Spectrometry Imaging of Three-dimensional Cell Cultures
- 07:49Conclusion
Immortalized cancer cell lines can be grown as 3D cell cultures, a valuable model for biological research. This protocol describes mass spectrometry imaging of 3D cell cultures, including improvements in the sample preparation platform. The goal of this protocol is to instruct users to prepare 3D cell cultures for mass spectrometry imaging analysis.
