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Bioengineering

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JoVE Journal Bioengineering
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Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models

Article DOI: 10.3791/52313-v • 08:14 min • December 5th, 2014
December 5th, 2014 •
1,2, 1,2, 1,2
1Department of Chemistry and Biochemistry, University of Notre Dame, 2Harper Cancer Research Institute, University of Notre Dame

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Summary
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Immortalized cancer cell lines can be grown as 3D cell cultures, a valuable model for biological research. This protocol describes mass spectrometry imaging of 3D cell cultures, including improvements in the sample preparation platform. The goal of this protocol is to instruct users to prepare 3D cell cultures for mass spectrometry imaging analysis.

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Sample Preparation Strategies Mass Spectrometry Imaging 3D Cell Culture Models Biological Research Cell Culture Spatial Complexity Molecular Complexity Tissue Cell Lines In Vitro Conditions Colon Cancer Cell Lines 3D Cell Culture Spheroids Avascular Tumor Nodules Immunohistochemistry Classical Imaging Methods Mass Spectrometric Imaging MALDI Mass Spectrometric Imaging Humans Animal Models Sample Preparation Strategies Growth Harvesting Sectioning Washing Analysis Matrix-assisted Laser Desorption/ionization-mass Spectrometry (MALDI-MS) Imaging Colon Carcinoma 3D Cell Cultures
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