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JoVE Journal
Immunology and Infection

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Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations
 

Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations

Article DOI: 10.3791/52351 09:25 min
January 10th, 2015

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Summary January 10th, 2015

Using optimized human peripheral blood mononuclear cell (PBMC) ex vivo assays, we showed that a combustible tobacco product preparation markedly suppresses receptor-mediated intracellularly secreted cytokines and cytolytic ability of effector PBMCs. These rapid assays may be useful in product evaluation and understanding the potential long-term effects of tobacco exposure.

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