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Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations
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January 10, 2015
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Using optimized human peripheral blood mononuclear cell (PBMC) ex vivo assays, we showed that a combustible tobacco product preparation markedly suppresses receptor-mediated intracellularly secreted cytokines and cytolytic ability of effector PBMCs. These rapid assays may be useful in product evaluation and understanding the potential long-term effects of tobacco exposure.
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Arimilli, S., Damratoski, B. E., G.L., P. Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations. J. Vis. Exp. (95), e52351, doi:10.3791/52351 (2015).
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