An <em>In Vitro</em> Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H<sub>3</sub> 5,10,15-tris(pentafluorophenyl)corroles

Grace Y. Tang; Melanie A. Pribisko; Ryan K. Henning; Punnajit Lim; John Termini; Harry B. Gray; Robert H. Grubbs

doi:10.3791/52355

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An In Vitro Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H₃ 5,10,15-tris(pentafluorophenyl)corroles

An <em>In Vitro</em> Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H<sub>3</sub> 5,10,15-tris(pentafluorophenyl)corroles

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

An In Vitro Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H₃ 5,10,15-tris(pentafluorophenyl)corroles

An In Vitro Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H₃ 5,10,15-tris(pentafluorophenyl)corroles

11,532 Views

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09:00 min

•

March 18, 2015

DOI:

10.3791/52355-v

DOI:

10.3791/52355-v

•

09:00 min

March 18, 2015

•

11513 Views

Grace Y. Tang¹, Melanie A. Pribisko¹, Ryan K. Henning¹, Punnajit Lim², John Termini², Harry B. Gray¹, Robert H. Grubbs¹

¹Division of Chemistry and Chemical Engineering,California Institute of Technology ²Department of Molecular Medicine,Beckman Research Institute of the City of Hope

Summary

Gallium(III) 5,10,15-(tris)pentafluorophenylcorrole and its freebase analogue exhibit low micromolar cell cytotoxicity. This manuscript describes an RNA transcription reaction, imaging RNA with an ethidium bromide-stained gel, and quantifying RNA with UV-Vis spectroscopy, in order to assess transcription inhibition by corroles and demonstrates a straightforward method of evaluating anticancer candidate properties.