Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

Keiji Itaka; Satoshi Uchida; Akitsugu Matsui; Kayoko Yanagihara; Masaru Ikegami; Taisuke Endo; Takehiko Ishii; Kazunori Kataoka

doi:10.3791/52384

Journal

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Bioengineering

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Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

8,739 Views

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07:40 min

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July 31, 2015

DOI:

10.3791/52384-v

DOI:

10.3791/52384-v

•

07:40 min

July 31, 2015

•

8722 Views

Keiji Itaka¹, Satoshi Uchida¹, Akitsugu Matsui¹, Kayoko Yanagihara¹, Masaru Ikegami¹, Taisuke Endo², Takehiko Ishii³, Kazunori Kataoka²

¹Graduate School of Medicine, Laboratory of Clinical Biotechnology,The University of Tokyo ²Graduate School of Engineering, Department of Materials Engineering,The University of Tokyo ³Graduate School of Engineering, Department of Bioengineering,The University of Tokyo

Summary

This protocol describes a cell transplantation system using genetically modified, injectable spheroids. Cell spheroids are cultured on micropatterned culture plates and recovered after gene introduction using polyplex nanomicelles. This system facilitates prolonged transgene expression from the transplanted cells in host animals while maintaining the innate function of the cells.