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JoVE Journal Chemistry
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A Colorimetric Assay that Specifically Measures Granzyme B Proteolytic Activity: Hydrolysis of Boc-Ala-Ala-Asp-S-Bzl

Article DOI: 10.3791/52419
November 28th, 2014 •
1, 1, 1
1Cancer Immunology Program, Peter MacCallum Cancer Centre

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November 28th, 2014

We describe a simple, quantitative colorimetric assay that specifically measures the proteolytic activity of human, mouse or rat Granzyme B (GzmB). This protocol can be easily adapted for determining protease activity of other granule serine proteases by the hydrolysis of other synthetic peptide substrates with an appropriate recognition sequence.

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