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Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS
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Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS

Article DOI: 10.3791/52424-v • 08:38 min • November 8th, 2015
November 8th, 2015 •
1, 1, 1, 1, 2, 3, 4, 1
1iC42 Clinical Research and Development, University of Colorado, Anschutz Medical Campus, 2Division of Clinical Pharmacology, Cincinnati Children's Hospital Medical Center, 3Food and Drug Administration (FDA), Center of Drug Evaluation Research - Office of Generic Drugs, 4Transplant Clinical Research, University of Cincinnati

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Here we describe a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay to quantify the immunosuppressant tacrolimus in dried blood spots using a simple manual protein precipitation step and online column extraction.

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Immunosuppressant Tacrolimus Dried Blood Spots LC-MS/MS Therapeutic Drug Monitoring Dose Adjustment Whole Blood Trough Concentrations Home Therapeutic Drug Monitoring Adherence Monitoring Finger Stick Filter Paper Analytical Laboratory High-performance Liquid Chromatography-tandem Mass Spectrometry (HPLC-MS/MS) Protein Precipitation Step Online Column Extraction 6-mm Disc Blood Spot Homogenization Methanol/0.2 M ZnSO4 Internal Standard D2,13C-tacrolimus Vortexing Centrifugation Online Extraction Column Formic Acid/acetonitrile Gradient
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