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Nucleocapsid Annealing-Mediated Electrophoresis (NAME) Assay Allows the Rapid Identification of HIV-1 Nucleocapsid Inhibitors
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Nucleocapsid Annealing-Mediated Electrophoresis (NAME) Assay Allows the Rapid Identification of HIV-1 Nucleocapsid Inhibitors

Nucleocapsid Annealing-Mediated Electrophoresis (NAME) Assay Allows the Rapid Identification of HIV-1 Nucleocapsid Inhibitors

DOI:
10.3791/52474-v

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08:33 min

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January 19, 2015

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, , ,
1Department of Pharmaceutical and Pharmacological Sciences,University of Padova, 2The RNA Institute,SUNY Albany

Chapters

  • 00:05Title
  • 02:03Controls Preparation
  • 03:24Sample Preparation to Analyze Anthraquinone Activity on NC and (12-55)NC
  • 06:25Results: Effects of Threading Intercalator 1 Assessed by NAME Assay
  • 07:15Conclusion

Summary

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This protocol describes NAME, an assay that allows the rapid identification of molecules able to inhibit in vitro the chaperone activities of HIV-1 nucleocapsid protein.

Tags

Keywords: Nucleocapsid Annealing-Mediated Electrophoresis (NAME) AssayHIV-1 Nucleocapsid Protein (NC)RNA/DNA FoldingAntiviral Drug DevelopmentNC Chaperone Activity InhibitionThreading IntercalatorPAGE Analysis

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