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Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer

Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer

DOI:
10.3791/52502-v

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09:56 min

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February 11, 2015

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, , , ,
1Center for Human Immunobiology,Texas Children’s Hospital, 2Department of Pediatrics,Baylor College of Medicine, 3Department of Pathology and Immunology,Baylor College of Medicine

Chapters

  • 00:05Title
  • 01:52Assembling the Glass-supported Planar Lipid Bilayer
  • 06:17Imaging of the NK Synapse on Lipid Bilayer Using Stimulated Emission Depletion (STED)
  • 08:11Results: Super-resolution Images of NK Cell Synapse on the Lipid Bilayer Using STED Microscope
  • 09:21Conclusion

Summary

Automatic Translation

Automatic Translation

We describe here a combination of the glass-supported lipid bilayer technique of forming immunological synapses with the super-resolution imaging technique of stimulated emission depletion (STED) microscopy. The goal of this protocol is to provide users with the instructions necessary to successfully carry out these two techniques.

Tags

Keywords: Super-resolution ImagingNatural Killer CellImmunological SynapseGlass-supported Planar Lipid BilayerSTED MicroscopyPerforinFilamentous ActinNK Cell Activation Receptor CD16

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