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Journal
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Immunology and Infection
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调查肥大细胞分泌颗粒;从合成到胞吐作用
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Immunology and Infection
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Immunology and Infection
Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
Please note that all translations are automatically generated.
Click here for the English version.
调查肥大细胞分泌颗粒;从合成到胞吐作用
DOI:
10.3791/52505-v
•
16:01 min
•
January 26, 2015
•
Nurit P. Azouz
2
,
Mitsunori Fukuda
,
Marc E. Rothenberg
,
Ronit Sagi-Eisenberg
1
Department of Cell and Developmental Biology
,
Sackler Faculty of Medicine, Tel Aviv University
,
2
Division of Allergy and Immunology, Department of Pediatrics
,
Cincinnati Children’s Hospital Medical Center, University of Cincinnati College of Medicine
,
3
Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences
,
Graduate School of Life Sciences, Tohoku University
Chapters
00:05
Title
02:37
Transfection of Rat Basophilic Leukemia (RBL) Cells
06:06
Measuring NPY mRFP Exocytosis
07:48
Time-lapse Microscopy of Exocytosis
10:51
Image Analyses
13:00
Results: Quantifying Mast Cells Secretory Granule (SG) Size and their Exocytosis
14:44
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本议定书的目标是开发一种方法,使肥大细胞分泌功能基因组分析。所述协议是基于荧光报道基因cotrasfected与感兴趣和实时的基因的释放定量评估分析分泌颗粒的形态。
Tags
Mast Cells
Secretory Granules
Exocytosis
Mediator Release
Regulated Secretion
Confocal Microscopy
Gene Expression
Molecular Mechanisms
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