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调查肥大细胞分泌颗粒;从合成到胞吐作用
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
DOI:

16:01 min

January 26, 2015

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Chapters

  • 00:05Title
  • 02:37Transfection of Rat Basophilic Leukemia (RBL) Cells
  • 06:06Measuring NPY mRFP Exocytosis
  • 07:48Time-lapse Microscopy of Exocytosis
  • 10:51Image Analyses
  • 13:00Results: Quantifying Mast Cells Secretory Granule (SG) Size and their Exocytosis
  • 14:44Conclusion

Summary

Automatic Translation

本议定书的目标是开发一种方法,使肥大细胞分泌功能基因组分析。所述协议是基于荧光报道基因cotrasfected与感兴趣和实时的基因的释放定量评估分析分泌颗粒的形态。

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